![]() ![]() Load with all the glassware and media filled upto 70%, of the chamber and the details are as follows.Diagram-1 Probe to 12 inside the chamber. ![]() ![]() ![]() All probes must reach temperature 121-124☌ and pressure must be within 15 to 18 lbs for 15min cycle.Record the temperature and lag time of each probe as per Annexure –1 & 2. by fixing all the 12 probes as per diagram-1. Carry out heat distribution studies by using a multi-point data logger and maintain holding time for 15 minutes at 15 lbs.The variation between the temperature of thermocouples and the standard thermometer found to be within the acceptance criteria.Calibrate all the thermocouples of data logger before and after the validation using standard thermometer and also made available party’s calibration certificates.Validation of the Autoclave is classified into the following.The following documents should be available:.Minimum annual maintenance schedule and inspection.Part 4 of the British Standard outlines the following as minimum maintenance routines for laboratory autoclaves:.Pour 1 liter of hot water down the drain to rinse out the detergent solution.Pour 1 liter of detergent and hot water solution down the drain with a funnel.If the instructions are unavailable, flush the exhaust line or chamber drain to keep it free of material that may interfere with air and steam leaving the chamber as follows: Weekly: Check the manufacturer’s instructions for maintenance of the exhaust line.Clean the shelves in the autoclave or the basket or cart that holds packs (including the wheels of the cart) with detergent and a cloth.Clean the door or lid gaskets with a cloth and check for defects.Do not use abrasive cleansers or steel wool, as these will scratch the surface and increase corrosion. Daily: Remove the outlet screen and clean with detergent and a brush under running water.General Guidelines for Routine Maintenance of Autoclaves Choose the correct conditions for your material.Be sure the autoclave is functioning properly before use.The term autoclave is also used to describe an industrial machine in which elevated temperature and pressure are used in processing materials. It was invented by Charles Chamberland in 1879,although a precursor known as the steam digester was created by Denis Papin in 1679. An autoclave is a device to sterilize equipment and supplies by subjecting them to high pressure steam at 121° C or more. ![]()
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